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The solutions will fluctuate but must be in the assortment of 103 -104 transformants/µg DNA. The male's forceps will turn into curved, when the females' forceps remain straight. When ascending, the tail props up the rear of the body, while 1 hind foot moves ahead and then swings to the other aspect to deliver assist as the other hind foot developments. If there are too a lot of to count then document "lawn". Compare the expansion on plates two and 4 and then make clear your response. three. The bacterium are not able to grow in the presence of the antibiotic ampicillin unless it consists of the plasmid, and so there will be no growth on the LB/Amp plate of the microbes without the plasmid. There was no fluorescence obvious when the UV mild was shined on the plasmid in the tube mainly because it is not the plasmid that reacts to the gentle but the protein generated when the gene in the plasmid is translated. On plate 4 any microorganisms that has been transformed by taking up the pGREEN plasmid is now ready to develop in the existence of the antibiotic given that the plasmid also contains the gene allowing for for antibiotic resistance