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Phylogenetic assessment of the interactions of mannose-distinct bulb-form lectin proteins in D. rotundata (pink), A. thaliana (blue), B. distachyon (inexperienced), and O. sativa (orange). Venn diagram exhibiting conserved and one of a kind genes at 1:1 correspondence amongst D. rotundata, Arabidopsis thaliana, Brachypodium distachyon, and Oryza sativa. Non-redundant Gene Ontology (GO) terms "intracellular organelle", "protein binding", and "ion binding" were being noticeably enriched among D. rotundata genes that confirmed no orthology to the other species, but not amongst the conserved genes (Additional documents 9, 10). D. rotundata genes devoid of orthologs in the other species incorporated sixty eight genes encoding proteins with lectin domains that are associated in defense against microbial pathogens, nematodes, and bugs, accounting for 31% of the 216 lectin-coding genes functionally annotated in D. rotundata. For 12,625 D. rotundata genes, no orthologs or paralogs were uncovered in B. distachyon, O. sativa, or A. thaliana, and 11,348 D. rotundata genes had no apparent homologs in any of the 6 species shown in Fig. 3a and Additional file eight. Of these 11,348 genes with out homologs, 3422 had been expressed in tuber tissues, a tissue type not shared with the other species examined. However, the intercourse-willpower process may possibly change between Dioscorea species (see beneath), so the transferability of intercourse-linked web site DNA markers from D. rotundata to other species should be dealt with in long run scientific tests

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